Molecular Devices SpectraMax® series microplate readers. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional. Figure 1. SpectraMax® ABS. 1. Parameter SpectraMax iD5 SpectraMax i3x SpectraMax. The percentage of viability of each treatment group in different. Flasks containing 100 mL of YM broth were inoculated with 100. At 48 h after transfection, the Nano-Glo Live Cell Assay reagent was added and the luciferase activity in the medium and cells were measured using a SpectraMax M4 (Molecular Devices, San Jose, CA, USA). 10. Three kits covering a wide range of dsDNA concentrations. We therefore gathered salient. . Examples: “Press carefully to engage,” “ Do not press down. The DQ Green BSA probe. Cleavage of the fluorescent NAG substrate (4-Methylumbelliferyl-2-acetamido-2-deoxy-β-D-Glucopyranoside) was measured for 20 min with 45 s intervals at 30°C in a Molecular Devices (San Jose, CA) SpectraMax M4 microplate reader. The UV‐Vis absorption spectra and cell viability were measured by a multi‐mode microplate reader (SpectraMax® M4, Molecular Devices LLC) using quartz cuvettes with 1‐cm path length (Starna Scientific Ltd. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. SpectraMax ABS Plus SpectraTest ABS2 v1. Bioz Stars score: 98/100, based on 1 PubMed citations. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. Background-subtracted data values (mean +/- SD) are graphed. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual- monochromator microplate reader platform offering a wide range of. Products : SpectraMax M2/M2e reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax 190 reader, VersaMax reader, Gemini EM reader, SpectraMax Plus 384 reader. Images were taken using a fluorescence microscope (Nikon 80i, Japan). Highly selective for dsDNA over ssDNA or RNA. The ELISA kits (Lianke Bio, China) were used to detect the concentration of specific cytokines. Devices SpectraMax® M4). Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, SpectraMax M4 reader, AquaMax washer, SpectraMax Plus 384 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax L reader, SpectraMax M5/M5e. With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. . The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Z’-LYTE® Assay using the Z'-LYTE® Tyr6 kit. Detection modalities are shown in Table 1-1. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. Calpain activity assay The cells were treated with different drugs for 24 hours and were resuspended in 100 μL extraction buffer and homogenized by pipetting. M2 M2e M3 M4 M5 M5e ABS1 Location: Reader Validation-Plate Abs A reminder about recertification was added. 5. AquaMax® 4000. 2. Products : SpectraMax M3 reader, SpectraMax M4 reader, LMax II (Obsolete), FlexStation 3 reader, SpectraMax L reader, SpectraMax M5/M5e reader How do I know if the LM-1 plate is off or on? The LM1 plate has a push button switch in a cupped-out area on one end. For the microspheres containing moxifloxacin used in vivo, the liquid hydrogel was presoaked in a 24 mg/mL aqueous solution of moxifloxacin hydrochloride. Results were expressed as the mean percentage of CV compared with NC group. RESEARCH PAPER Effect of particle size and surface charge of nanoparticles in penetration through intestinal mucus barrier Sony Priyanka Bandi & Yashada Sanjay Kumbhar & Venkata Vamsi Krishna VenugantiThe Fibrinogen standard curve was prepared as described in Section 10. In. 305 reference level saturation. Simulated Exposure SystemOptical density readings were obtained at various timepoints from 0 to 128 hours following inoculation using a SpectraMax M4 spectrophotometer (Molecular Devices, San Jose, CA). Statistical analysis. 2. Using cross-feeding bioassay, a functional LuxI/LuxR-type acyl-homoserine lactone (AHL)-mediated quorum sensing (QS) system. G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax i3x, SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through G08forSpectraMaxLinstrument Cross-talk D09 through D11,E09 through E11,andF09 through F11 Linearity G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax. These samples were analyzed using a multimode plate reader (SpectraMax M4, Molecular Devices, USA). It should work on the Spectramax L reader and also on the Spectramax M3, M4, and M5 plate readers (new firmware only) with Softmax Pro ® software version 5. The IC50 curves were calculated using variable slope for log (inhibitor) vs. D 1 1. Cell viability was calculated using the following formulaThe luciferase signal was read in a plate reader (SpectraMax M4; Molecular Devices, Sunnyvale, CA). 1. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. The combination index (CI) was calculated. To quantify F-CHP fluorescence, stained samples were digested in 500 µl of 1 mg/ml proteinase K in water for 3 hours at 60 °C. Thirty min later, each well was homogenized and the optical density (OD) was measured in a spectrometer (Spectramax M4, Molecular Devices) at 570 nm. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature. The medium was then removed from the wells, and 100 µl of DMSO was added to solubilize the formazan crystals. FlexStation ®, SpectraMax. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. The absorbance of the biofilm was measured at 595 nm using a microplate reader (SpectraMax M4) and the results were expressed as percentage changes with respect to the control (without compounds). Products : SpectraMax M2/M2e reader, SpectraMax Plus 384 reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, SpectraMax M3 reader Can you recommend disposable cuvettes for use in the microplate readers? Molecular Devices® does not recommend any specific disposable cuvette brands. WARNING! The instrument weighs approximately 36 pounds (16. No. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. Measurements of temperature increase (ΔT) during the exposure of MNP/AMF hyperthermiaThe culture medium was removed, and the blue product was dissolved in DMSO (100 μl). SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. item 7 replacement bulb for molecular devices spectramax m4 flash lamp replacement bulb for molecular devices spectramax m4 flash lamp. "With the launch of the Molecular Devices® SpectraMax® M3 and M4 Multi-Mode Microplate Readers, we now offer a full range of multi-mode detection capabilities within our SpectraMax® product line," said Andy Boorn, President of MDS Analytical Technologies. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. SpectraMax M Series Multi-Mode Microplate Readers | Molecular Devices · 2020-04-14 · use two scanning monochromators so the user can determine optimal excitation and emission settings, of 8 /8 Match case Limit results 1 per pageThe culture medium was removed, and the blue product was dissolved in DMSO (100 μl). MEK Inhibition Induces Therapeutic Iodine Uptake in a Murine Model of Anaplastic Thyroid Cancer Oussama ElMokh1, Vincent Taelman2, Piotr Radojewski2, Matthias A. DNA standards. 2. . SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. The fluorescence intensity was measured as ratio of values at an excitation at 340 nm and 380 nm with an emission at 510 nm, using Spectramax M4 (Molecular Devices). Assessment of Neurite Outgrowth. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellSpectraMax® M4 Microplate Reader Molecular Devices LLC Applications: Absorbance, Cell Health, Cell Viability, Cellular. +1 250-415-0785. The instrument reads all Plate sections in the experiment. ATP concentration (M) Average RLU SpectraMax L SpectraMax i3x (cartridge) SpectraMax Mini SpectraMax iD5 SpectraMax i3x (onboard) SpectraMax M5eThe SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. Preconfigured SoftMax ® Pro Software protocols (available at softmaxpro. SpectraMax® ABS Plus. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax. Multi-Mode Microplate Readers. Published Dec 16, 2020 | Updated Dec 16, 2020. 11. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. In luminescence mode, if you are specifying a wavelength for the read, you can select an emission bandwidth of either 15 nm or 25 nm. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v1. The fluorescent signal was measured by multiplate reader (Spectramax M4, Molecular Devices LLC, Sunnyvale, California, USA). For a complete list of the instruments supported by this release of the SoftMax Pro Software, see Supported Instruments on page 111. Molecular Devices SpectraMax ® M3/M4/M5/M5e Multi-Mode Microplate Readers + + Molecular Devices SpectraMax. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. The chamber contains springs that position the cuvette in the proper alignment for a read. Note: In this user guide, all references to SpectraMax Multi-Mode. The luciferase values were determined by normalization to Renilla values (as a control) to correct for variation in transfection efficiencies. Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. MEK Inhibition Induces Therapeutic Iodine Uptake in a Murine Model of Anaplastic Thyroid Cancer Oussama ElMokh1, Vincent Taelman2, Piotr Radojewski2, Matthias A. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode. Technical specifications Detection limit for ATP Glow 0200-7012 (LUM) Optimized Guaranteed* 96-well (200 µL) 1. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. *Statistical significance: p < 0. 0 Micromoles per Joule, ensuring the highest light output using the least amount of electricity. . Results were expressed as a percentage relative to the control group (defined as 100%). Example: [Drawer]. Description 1. 5 mg mL −1 with 0. SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise: Trinean: DropSense96: AMBIENT TEMPERATURE STORAGE: Liconic: LPX 220 Hotel: LPX 440 Hotel: Thermo Fisher Scientific: Cytomat 10 Hotel: Cytomat 2 Hotel: Cytomat 24 Hotel: Cytomat 8 Linear Hotel: Cytomat 9 Hotel (MPH) BAR CODE READING: FluidX: Perception (Vial. Dual monochromators for assay flexibility With SpectraMax M Series readers, there is no need to utilize expensive filters to optimize detection levels and background. 4) In vivo pharmacokinetic studies. The following document is intended to demonstrate setup of this instrument. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of. SpectraMax® L (software version 5. 3. August 16, 2017. Description 1. Images were taken using a fluorescence microscope (Nikon 80i, Japan). Here, we present an approach to predict UV-visible absorption spectra of solvated aromatic molecules by quantum chemistry (QC) and machine learning (ML). The SpectraMax M2 and M2e are versatile and reliable. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. SpectraMax® Aquamax. For more detailed information and technical support of Molecular Devices instruments or software, Luciferase Detection Instrumentation Guide. In the competitive field of drug development where timely go/no-go decisions must be made to bring effective class-leading drugs to market, it is vital to work with a CRO that can provide highly. 次いで、ユーザーのラボに. 4. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence polarization, and time-resolved. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. The whole process was protected from direct light. e. Like all SpectraMax® Readers, the new M3 and M4 readers leverage patented technologies to deliver added value to users. Products : SpectraMax M2/M2e reader, SpectraMax Plus 384 reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, SpectraMax M3 reader Where can I find UV/Vis Plastic Disposable Cuvettes? Some Plastic Disposable UV (300 nm +) and VIS Cuvette sources: NOTE: Some disposables with channel sides (as opposed to full. The chloride and pH. NCI-60 cell panel screening was performed by the NCI/National Institutes of Health developmental therapeutics program (Bethesda, US). The fluorescence intensity was measured as ratio of values at an excitation at 340 nm and 380 nm with an emission at 510 nm, using Spectramax M4 (Molecular Devices). Resolution: In the GxP audit trail, the SpectraMax M3 and M4 Readers are now identified correctly. MTT was then removed, and the formed formazan crystals were then dissolved in 150‐μl DMSO. AquaMax® 2000. TEM. SpectraMax M2 and M2e Performance Specifications SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. Bottom-reading is usually recommended for cell-based assay with adherent cells. 025 mol/L sulfuric acid) and then measured following the molybdenum antimony colorimetric method (multifunctional microplate reader, SpectraMax M4, MD company, USA). 73. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. of a 96 well-plate and cultured for 24h at 37 °C. Fluorescence Microplate Reader Comparison Chart. Calpain activity assay The cells were treated with different drugs for 24 hours and were resuspended in 100 μL extraction buffer and homogenized by pipetting. ZERO BIAS - scores, article reviews, protocol conditions and more7. Click Read. The use of gene reporters such as luciferase permits highly sensitive and nondestructive monitoring of gene expression. The sample absorbance was read at 450 nm using a SpectraMax M4 spectrophotometer (Molecular Devices, Sunnyvale, CA, USA). 95. ELISA standard curves were generated using a four-parameter logistic curve model (GraphPad Prism 8, GraphPad Software; San Diego, CA, USA). The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). VIEW DETAILS. 39. Standard curves for quantifying samples were generated by using serial dilutions of BSA from 0 to 0. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. : 915 515 403 • Fax: 914 334 545 • e-mail: info@bionova. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. We therefore gathered salient. The optical absorbance of the plate at 450 nm was measured using a multifunctional microplate reader SpectraMax M4 (Molecular Devices, LLC, San Jose, CA, USA). 22 μM was mixed with 10-μl enzyme giving a final enzyme concentration in the. 01) + 0. For labeling representative cells, they were treated under the same conditions as above, followed by fixation with 2% paraformaldehyde for 20 min at room temperature. The absorption value at 570 nm was measured using a Molecular Devices SpectraMax M4 plate reader, and the absorbance at 690 nm was subtracted as the background. Data was calculated subtracting OD440 recorded with the final OD600 values. The M4 Reader offers the same modes as the M3 Reader, plus time-resolved fluorescence, and can be upgraded to include fluorescence polarization. 0 compensates for most changes in environmental temperature or pressure. Background-subtracted data values (mean +/- SD) are graphed. spr SpectraMax Plus 384. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. After 7 days of culture, the colonies generated. Methods for the accurate and rapid screening of IVT conditions will facilitate RNA polymerase engineering, promoter optimization, and screening for new transcription inhibitor drugs. 4 kilograms). The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. SpectraMax® M4 Multi-Mode Microplate Reader MLDVM4To quantify F-CHP fluorescence, stained samples were digested in 500 μl of proteinase K (1 mg/ml) in water for 3 hours at 60°C. Our absorbance plate readers feature our PathCheck Sensor. Danaher Inc spectramax m series Spectramax M Series, supplied by Danaher Inc, used in various techniques. FlexStation ®, SpectraMax. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through G08forSpectraMaxLinstrument Cross-talk D09 through D11,E09 through E11,andF09 through F11 for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. There are multiple vendors. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. With optimized reagents, option to operate the. The fluorescent signal at an excitation wavelength of 530 nm and emission wavelength of 580 nm was measured by Spectramax M4, USA. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. Serum C-terminal telopeptide of type I collagen (S-CTX), osteocalcin (BGP), and tartrate-resistant acid phosphatase (TRAP) levels in serum and the receptor activator of the NF-κB ligand (RANKL), osteoprotegerin (OPG), and RANK levels in marrow were determinedThe ELISA kits (Lianke Bio, China) were used to detect the concentration of specific cytokines. Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. CMC values were determined from the pyrene fluorescence in solution as a function of conjugate. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. The SpectraMax®M3, M4, M5, and M5eMicroplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. 1 Figure from 528 Publications Molecular Devices LLC. Molecular Devices SpectraMax M4 upgradable microplate/cuvette reader (90V to 240V) with current version of SoftMax Pro. • Tel. Formazan crystals formed were solubilized in 10 μl DMSO, and absorbance was recorded using a multimode plate reader (SpectraMax M4; Molecular Devices, USA) at 606-nm wavelength. 2. Plates were then read at 450 nm using a SpectraMax M4 device (Molecular Devices, Sunnyvale, CA). MIC assay: After diluting colloidal nanoparticle solutions of varying concentrations in TSB, they were injected onto a 96-well plate with a strain of bacteria at a concentration of 10 6 CFU/mL. . All associated StakMax® stackers. 315 can't find zero order. 2 pM 2 pM 384-well (50 µL) 3 pM 4 pMCell viability was determined by addition of MTT ([3-(4,5)-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide, Sigma) to cells and incubation for 4 h. SPECTROMAXx with iCAL 2. SpectraMax M3 Reader SpectraMax M4 Reader SpectraMax M5 Reader SpectraMax M5e Reader 5 B > 4 4 5 B 5 : F 8 8 > 3 ; > I 5 = 8 5. For the FlexStation 3 Reader - Keep the Black Plate Adapter in place fro both Top and Bottom reads. Exhibit with us {{>currencyLabel}} Back. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. 1. Large molecule bioanalysis, immunogenicity and PK testing with higher quality data, rapid turnaround and fewer surprises. , USA). All experiments were performed in triplicate. Standard features include a cuvette port,. Hemoglobin release from erythrocyte lysis was detected by measuring the optical density (OD) of the supernatant at 412 nm using a microplate reader (SpectraMax M4 from Molecular Devices, or Tecan SPARK, from Tecan, Männedorf, Switzerland). Transfer plate to SpectraMax M5 with excitation monochromator set to 455 nm with 10 nm bandwidth, emission monochromator set to 525 nm with 10 nm bandwidth, and 515 nm cutoff filter. Molecular Devices SpectraMax ® M3/M4/M5/M5e Multi-Mode Microplate Readers + + Molecular Devices SpectraMax. Italic and boldface type are used for emphasis. Highly selective for dsDNA over ssDNA or RNA. Plus 384 ABS1 Location: Reader Validation-Plate Abs A reminder about recertification was added. Louis, MO, USA). Results were normalized and expressed as a percentage of live cells. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi-mode. The absorption value at 570 nm was measured using a Molecular Devices SpectraMax M4 plate reader, and the absorbance at 690 nm was subtracted as the background. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. The Dagda Lab is a mitochondrial biology-oriented research group located at the University of Nevada, Reno School of Medicine that currently spearheads basic and translational research projects supported. We would like to show you a description here but the site won’t allow us. . The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. 2. 05 mg mL −1. /525 em. ). Choose from a three- (M3), four- (M4), orThe optical density was determined spectrophotometrically at 405 nm using a SpectraMax M4 microplate reader (Molecular Devices, Sunnyvale, CA). Shop our huge selection of OEM, MRO, construction, industrial, and safety products. Background-subtracted data values (mean +/- SD) are graphed. . The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. spr SpectraMax iD5 SpectraTest ABS2 v1. The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science. FlexStation™ III. Ninety microliters of 2-chloro-4-nitrophenyl-α- d -maltotrioside substrate dilutions from 5000 to 1. Microplate Reader Shelf. Introduction. Instruments Ltd. to View Figures. book Page 1 Wednesday, July 7, 2010 2:09 PM SpectraMaxM3,M4,M5,andM5eMicroplateReaderUserGuide 8 0112-0115G CAUTION!Useoforganicsolventscancauseharmtotheopticsintheinstrument. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). Living cells were then incubated with 100 µl of medium assay provided by the assay kit. 116 SpectraMax M5 and M5e Multi-Mode. All configurations. However, whether the Rhizoma drynariae total flavonoids (RDTF) can promote the absorption of calcium and enhance the bone formation is unclear. Diclofenac potassium at non-toxic concentration on U87-MG was used as positive control. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Images were visualized using a fluorescence microscope (Nikon, Japan) . Pentobarbital sodium and N,N,N′,N′-tetramethylethylenediamine (TEMED) were purchased from Sigma-Aldrich (St. 2. 12. e. The lower critical solution temperature (LCST) at which phase transition occurs was measured via plate reader absorbance for n = 3 samples (SpectraMax M4; Molecular Devices). Instruments and Settings for Fluorescence Read-out (Other Fluorescent Assays) FLUOstar Omega, VANTAstar, CLARIOstar, PHERAstar, PolarStar Optima. SpectraMax M Series Multi-Mode Microplate Readers | MolecularThe SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. alamarBlue reagent volume in a different spheroid microplate formatA SpectraMax M4 microplate reader (Molecular Devices, LLC, Sunnyvale, CA, USA) was used to measure the absorbance at a wavelength of 450 nm. 0. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-Mode MicroplateReader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5e. Following incubation, the media was aspirated completely and MTT formazan crystals formed were dissolved by the addition of dimethylsulfoxide, and the reading was taken at 570 nm using SpectraMax M4. neoformans was cultured from glycerol stock in 5 mL of YM broth at 25°C for 72 hours at 200 rpm. The absorbance was determined by a microplate reader (SpectraMax M4, USA) at 405 nm. Dettmer4, Wayne A. Cet appareil ISM est confomre à la norme NMB-001 du Canada. 22 μM was mixed with 10-μl enzyme giving a final enzyme concentration in the. Choose from a three- (M3), four- (M4), or Qualify the luminescence performance of the SpectraMax . Bio-layer interferometry assays The binding kinetics of 1H1 to spike or RBD proteins from SARS-CoV-2 variants was performed by biolayer interferometry analysis on the GatorPrime Label-Free Bioanalysis. Size and zeta potential of nanoparticles were measured by Zetasizer Nano ZS90 (Malvern Instrument). 2. D 47 7. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). 9 pg/mL. Description 1. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Cellular Reactive Oxygen Species (ROS) was measured in Neuro2a cells seeded in 6-well plate at a density of 5000 cells/well. Spectramax M4 Multi mode Microplate Detection System: Benchpro 4100 instrument card processing station: Muse Cell Analyzer: Veriti 96w Thermal Cycler: Small Animal Anaesthesia System: HPLCs (Analytical & Preparative) ACQUITY UPLC H-Class Bio: LC-MS/MS Q-tof 6540: Gas Chromatograph: GC-2014: Automatic Digital Polarimeter: FT-IR. instruments you must first insert an adapter plate. Like all. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Statistical. Microscopic evaluation of apoptosis. A microplate reader (SpectraMax M4; Molecular Devices) was used to measure the absorbance (Abs) at 545 mM and the hemolysis rate was calculated as follows: (Eq. Lastly, the TMB substrate (Beyotime) was added, and absorbance was measured at 450 nm by a microplate reader (SpectraMax M4). Description 1. Operated from(A) ROS levels and (B) NAD+/NADH ratios in rotenone-pretreated young HDFs. Then, the fluorescence intensity was quantified by using a spectrophotometer (Spectramax M4, MolecularDevices, San Jose, CA, USA) at λ ex: 579 nm and λ em: 599 nm. Consult Molecular Devices's entire SpectraMax M series catalogue on MedicalExpo. Measurement of Mitochondrial Membrane Potential by JC-1. 6. com. 0 statistics software. In. Fluorescence Microplate Reader Comparison Chart. For a complete list of the instruments supported by this release of the SoftMax Pro Software, see Supported Instruments on page 111. 2. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. /600 em. SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise: Trinean: DropSense96: AMBIENT TEMPERATURE STORAGE: Liconic: LPX 220 Hotel: LPX 440 Hotel: Thermo Fisher Scientific: Cytomat 10 Hotel: Cytomat 2 Hotel: Cytomat 24 Hotel: Cytomat 8 Linear Hotel: Cytomat 9 Hotel (MPH) BAR CODE. Roelli1, Amandine Stoss1, Rebecca A. ). Host your own live radio show with. The SpectraMax M4 reader had identical performance specifications to the SpectraMax M5 reader for absorbance and fluorescence detection. 7. We have two NanoDrop micro-scale spectrophotometers, a Molecular Devices Spectramax M4 micro plate reader and a UVP gel documentation camera. Environmental Chamber and Respiratory SimulatorsRhizoma drynariae, a traditional Chinese herb, is commonly used in treatment of bone healing in osteoporotic fractures. Optical density readings were obtained at various timepoints from 0 to 128 hours following inoculation using a SpectraMax M4 spectrophotometer (Molecular Devices, San Jose, CA). 4 Cell viability assay The colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was employed to determine cell viability; 1. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. The ELISA kits (Lianke Bio, China) were used to detect the concentration of specific cytokines. Fluorescence intensities of each well were examined by a microplate reader (SpectraMax M4, Molecular Devices, CA, USA) with 485-nm excitation and 525-nm emission wavelengths. S. Description 1. To report an issue with this product, Brief content visible, double tap to read full content. Turn the instrument around so that the back of the instrument is. At sacrifice, serum was isolated from mouse total blood and maintained at –80 °C. 005) Photometric PrecisionThe SpectraMax Paradigm Multi-Mode Detection Platform is a modular multi-mode microplate detector. 38. SpectraMax ABS/ABS Plus SpectraMax i3/i3x MiniMax SpectraMax iD3/iD5 Spectramax 190 EXT Spectramax 340PC/384 Spectramax Plus 384 Spectramax L Spectramax M2 Spectramax M2e Spectramax M3 Spectramax M4 Spectramax M5 Spectramax M5e Spectramax Mini Versamax EXT Vmax Microplate Washers; Instrument Field Depot; Aquamax 2000 Aquamax 2000/Stakmax. SpectraMax M2 and M2e Performance Specifications SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. Fastenal is the largest fastener distributor in North America. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. TSQ was dissolved in Lock’s buffer (pH 7. If you need to relocate a SpectraMax Multi-Mode Microplate Reader, follow these steps. † Bottom mounting for SpectraMax Paradigm Readers only. Table 2. Compare Fluorescence Microplate Readers from leading suppliers on Biocompare. Cell uptake studies. 5 mg mL −1 with 0. 0. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. The conjugate was also characterized using FTIR (FT/IR-4200, Jasco Inc. Hexokinase reagent (Eagle Diagnostics) was added and the reaction absorbance was read at 340 nm using a SpectraMax M4 multimode plate reader (Molecular Devices). 4. The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and either 15 nm or 25 nm for emission. The SpectraMax M4 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96 and 384. data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. Each well was scanned at nine evenly distributed points and averaged automatically with the software. 1. Large Molecule Bioanalysis. neoformans was cultured from glycerol stock in 5 mL of YM broth at 25°C for 72 hours at 200 rpm. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Published Dec 16, 2020 | Updated Jan 25, 2021 Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, Gemini XPS reader, SpectraMax M5/M5e reader, SpectraMax.